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human healthy primary pulmonary arterial endothelial cells paecs  (PromoCell)


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    PromoCell human healthy primary pulmonary arterial endothelial cells paecs
    Figure 2. PTPN1 deficiency induced <t>endothelial</t> dysfunction in <t>PAECs.</t> (A) siRNA-mediated knockdown of PTPN1 showed decreased PAEC viability, as assessed by MTT assay and hemocytome- ter countings. (B) PTPN1 silencing induced apoptosis, as evidenced by increased caspase 3/7 levels in PAECs. (C) PTPN1 silencing decreased ability of PAEC tube formation in a Matrigel tube formation assay. Angiogenesis was quantified in the images using ImageJ software. Total tube lengths were presented in µm. The number of nodes were counted in the analyzed area. Scale bar = 1000 µm. Data represented as mean ± SEM (n = 3–6), student t-test. * p < 0.05, *** p < 0.001.
    Human Healthy Primary Pulmonary Arterial Endothelial Cells Paecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+healthy+primary+pulmonary+arterial+endothelial+cells+paecs/Human+Pulmonary+Microvascular+Endothelial+Cells/pm36672250-52-0-11
    Average 96 stars, based on 162 article reviews
    human healthy primary pulmonary arterial endothelial cells paecs - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension."

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.

    Journal: Cells

    doi: 10.3390/cells12020316

    Figure 2. PTPN1 deficiency induced endothelial dysfunction in PAECs. (A) siRNA-mediated knockdown of PTPN1 showed decreased PAEC viability, as assessed by MTT assay and hemocytome- ter countings. (B) PTPN1 silencing induced apoptosis, as evidenced by increased caspase 3/7 levels in PAECs. (C) PTPN1 silencing decreased ability of PAEC tube formation in a Matrigel tube formation assay. Angiogenesis was quantified in the images using ImageJ software. Total tube lengths were presented in µm. The number of nodes were counted in the analyzed area. Scale bar = 1000 µm. Data represented as mean ± SEM (n = 3–6), student t-test. * p < 0.05, *** p < 0.001.
    Figure Legend Snippet: Figure 2. PTPN1 deficiency induced endothelial dysfunction in PAECs. (A) siRNA-mediated knockdown of PTPN1 showed decreased PAEC viability, as assessed by MTT assay and hemocytome- ter countings. (B) PTPN1 silencing induced apoptosis, as evidenced by increased caspase 3/7 levels in PAECs. (C) PTPN1 silencing decreased ability of PAEC tube formation in a Matrigel tube formation assay. Angiogenesis was quantified in the images using ImageJ software. Total tube lengths were presented in µm. The number of nodes were counted in the analyzed area. Scale bar = 1000 µm. Data represented as mean ± SEM (n = 3–6), student t-test. * p < 0.05, *** p < 0.001.

    Techniques Used: Knockdown, MTT Assay, Tube Formation Assay, Software

    Figure 3. PTPN1 is downregulated in healthy human PAECs exposed to hypoxia and in the lung sugen5416/hypoxia-induced PH rats. (A) 150,000 PAECs were seeded onto 6-well plates and exposed to 72 h of hypoxia. After that, PTPN1 expression was measured by qRT-PCR. Induction of hypoxia was verified by measuring VEGF expression by qRT-PCR [20]. (B,C) PTPN1 mRNA and protein expression was also measured in the lung of sugen5416/hypoxia rate models by qRT-PCR and western blotting (please see the PH model description and phenotypes data in [8]). Data are represented as mean ± standard error mean, n = 3–5; student t-test was performed to compare data between two samples. **** p < 0.0001. ns, not significant.
    Figure Legend Snippet: Figure 3. PTPN1 is downregulated in healthy human PAECs exposed to hypoxia and in the lung sugen5416/hypoxia-induced PH rats. (A) 150,000 PAECs were seeded onto 6-well plates and exposed to 72 h of hypoxia. After that, PTPN1 expression was measured by qRT-PCR. Induction of hypoxia was verified by measuring VEGF expression by qRT-PCR [20]. (B,C) PTPN1 mRNA and protein expression was also measured in the lung of sugen5416/hypoxia rate models by qRT-PCR and western blotting (please see the PH model description and phenotypes data in [8]). Data are represented as mean ± standard error mean, n = 3–5; student t-test was performed to compare data between two samples. **** p < 0.0001. ns, not significant.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Figure 4. PTPN1 is downregulated in the blood but not in the PAECs of PAH patients. We ana- lyzed RNA-seq data of PTPN1 expression in the whole blood ((n = 72 healthy and n = 359 PAH), for subject characteristics, please see [22]) (A) and in PAECs (n = 9/group, (GSE0126262, [23])) of PAH patients (B). TPM values for PTPN1 expression is shown in the graphs. PTPN1 was correlated with expression of BMPR2, SMAD5, and SMAD9 in healthy and PAH PAECs (GSE0126262) (C–E).
    Figure Legend Snippet: Figure 4. PTPN1 is downregulated in the blood but not in the PAECs of PAH patients. We ana- lyzed RNA-seq data of PTPN1 expression in the whole blood ((n = 72 healthy and n = 359 PAH), for subject characteristics, please see [22]) (A) and in PAECs (n = 9/group, (GSE0126262, [23])) of PAH patients (B). TPM values for PTPN1 expression is shown in the graphs. PTPN1 was correlated with expression of BMPR2, SMAD5, and SMAD9 in healthy and PAH PAECs (GSE0126262) (C–E).

    Techniques Used: RNA Sequencing, Expressing

    Figure 5. Correlation of PTPN1 expression with SMAD1 and ID1 in PAECs collected from healthy and PAH patients (GSE0126262). PTPN1 expression was not significantly correlated with SMAD1 (A) and ID1 (B) in the PAECs of PAH patients and healthy controls.
    Figure Legend Snippet: Figure 5. Correlation of PTPN1 expression with SMAD1 and ID1 in PAECs collected from healthy and PAH patients (GSE0126262). PTPN1 expression was not significantly correlated with SMAD1 (A) and ID1 (B) in the PAECs of PAH patients and healthy controls.

    Techniques Used: Expressing

    Related Articles

    Knockdown:

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).. Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281). .. C

    MTT Assay:

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).. Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281). .. C

    Tube Formation Assay:

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).. Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281). .. C

    Software:

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).. Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281). .. C

    Expressing:

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).. Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281). .. C

    Quantitative RT-PCR:

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).. Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281). .. C

    Western Blot:

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).. Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281). .. C

    RNA Sequencing:

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).. Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).Cells were grown in standard endothelial growth medium (Cat # C-22120; PromoCell GmbH) with growth factors supplementation and 100 U/mL penicillin–streptomycin solution (Gibco) under standard conditions (37 ◦C, 5% CO2, 21% O2, 90% humidity).

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension
    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281). .. C



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    human healthy primary pulmonary arterial endothelial cells paecs  (PromoCell)
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    PromoCell human healthy primary pulmonary arterial endothelial cells paecs
    Figure 2. PTPN1 deficiency induced <t>endothelial</t> dysfunction in <t>PAECs.</t> (A) siRNA-mediated knockdown of PTPN1 showed decreased PAEC viability, as assessed by MTT assay and hemocytome- ter countings. (B) PTPN1 silencing induced apoptosis, as evidenced by increased caspase 3/7 levels in PAECs. (C) PTPN1 silencing decreased ability of PAEC tube formation in a Matrigel tube formation assay. Angiogenesis was quantified in the images using ImageJ software. Total tube lengths were presented in µm. The number of nodes were counted in the analyzed area. Scale bar = 1000 µm. Data represented as mean ± SEM (n = 3–6), student t-test. * p < 0.05, *** p < 0.001.
    Human Healthy Primary Pulmonary Arterial Endothelial Cells Paecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+healthy+primary+pulmonary+arterial+endothelial+cells+paecs/Human+Pulmonary+Microvascular+Endothelial+Cells/pm36672250-52-0-11
    Average 96 stars, based on 1 article reviews
    human healthy primary pulmonary arterial endothelial cells paecs - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

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    Figure 2. PTPN1 deficiency induced endothelial dysfunction in PAECs. (A) siRNA-mediated knockdown of PTPN1 showed decreased PAEC viability, as assessed by MTT assay and hemocytome- ter countings. (B) PTPN1 silencing induced apoptosis, as evidenced by increased caspase 3/7 levels in PAECs. (C) PTPN1 silencing decreased ability of PAEC tube formation in a Matrigel tube formation assay. Angiogenesis was quantified in the images using ImageJ software. Total tube lengths were presented in µm. The number of nodes were counted in the analyzed area. Scale bar = 1000 µm. Data represented as mean ± SEM (n = 3–6), student t-test. * p < 0.05, *** p < 0.001.

    Journal: Cells

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.

    doi: 10.3390/cells12020316

    Figure Lengend Snippet: Figure 2. PTPN1 deficiency induced endothelial dysfunction in PAECs. (A) siRNA-mediated knockdown of PTPN1 showed decreased PAEC viability, as assessed by MTT assay and hemocytome- ter countings. (B) PTPN1 silencing induced apoptosis, as evidenced by increased caspase 3/7 levels in PAECs. (C) PTPN1 silencing decreased ability of PAEC tube formation in a Matrigel tube formation assay. Angiogenesis was quantified in the images using ImageJ software. Total tube lengths were presented in µm. The number of nodes were counted in the analyzed area. Scale bar = 1000 µm. Data represented as mean ± SEM (n = 3–6), student t-test. * p < 0.05, *** p < 0.001.

    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).

    Techniques: Knockdown, MTT Assay, Tube Formation Assay, Software

    Figure 3. PTPN1 is downregulated in healthy human PAECs exposed to hypoxia and in the lung sugen5416/hypoxia-induced PH rats. (A) 150,000 PAECs were seeded onto 6-well plates and exposed to 72 h of hypoxia. After that, PTPN1 expression was measured by qRT-PCR. Induction of hypoxia was verified by measuring VEGF expression by qRT-PCR [20]. (B,C) PTPN1 mRNA and protein expression was also measured in the lung of sugen5416/hypoxia rate models by qRT-PCR and western blotting (please see the PH model description and phenotypes data in [8]). Data are represented as mean ± standard error mean, n = 3–5; student t-test was performed to compare data between two samples. **** p < 0.0001. ns, not significant.

    Journal: Cells

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.

    doi: 10.3390/cells12020316

    Figure Lengend Snippet: Figure 3. PTPN1 is downregulated in healthy human PAECs exposed to hypoxia and in the lung sugen5416/hypoxia-induced PH rats. (A) 150,000 PAECs were seeded onto 6-well plates and exposed to 72 h of hypoxia. After that, PTPN1 expression was measured by qRT-PCR. Induction of hypoxia was verified by measuring VEGF expression by qRT-PCR [20]. (B,C) PTPN1 mRNA and protein expression was also measured in the lung of sugen5416/hypoxia rate models by qRT-PCR and western blotting (please see the PH model description and phenotypes data in [8]). Data are represented as mean ± standard error mean, n = 3–5; student t-test was performed to compare data between two samples. **** p < 0.0001. ns, not significant.

    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Figure 4. PTPN1 is downregulated in the blood but not in the PAECs of PAH patients. We ana- lyzed RNA-seq data of PTPN1 expression in the whole blood ((n = 72 healthy and n = 359 PAH), for subject characteristics, please see [22]) (A) and in PAECs (n = 9/group, (GSE0126262, [23])) of PAH patients (B). TPM values for PTPN1 expression is shown in the graphs. PTPN1 was correlated with expression of BMPR2, SMAD5, and SMAD9 in healthy and PAH PAECs (GSE0126262) (C–E).

    Journal: Cells

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.

    doi: 10.3390/cells12020316

    Figure Lengend Snippet: Figure 4. PTPN1 is downregulated in the blood but not in the PAECs of PAH patients. We ana- lyzed RNA-seq data of PTPN1 expression in the whole blood ((n = 72 healthy and n = 359 PAH), for subject characteristics, please see [22]) (A) and in PAECs (n = 9/group, (GSE0126262, [23])) of PAH patients (B). TPM values for PTPN1 expression is shown in the graphs. PTPN1 was correlated with expression of BMPR2, SMAD5, and SMAD9 in healthy and PAH PAECs (GSE0126262) (C–E).

    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).

    Techniques: RNA Sequencing, Expressing

    Figure 5. Correlation of PTPN1 expression with SMAD1 and ID1 in PAECs collected from healthy and PAH patients (GSE0126262). PTPN1 expression was not significantly correlated with SMAD1 (A) and ID1 (B) in the PAECs of PAH patients and healthy controls.

    Journal: Cells

    Article Title: PTPN1 Deficiency Modulates BMPR2 Signaling and Induces Endothelial Dysfunction in Pulmonary Arterial Hypertension.

    doi: 10.3390/cells12020316

    Figure Lengend Snippet: Figure 5. Correlation of PTPN1 expression with SMAD1 and ID1 in PAECs collected from healthy and PAH patients (GSE0126262). PTPN1 expression was not significantly correlated with SMAD1 (A) and ID1 (B) in the PAECs of PAH patients and healthy controls.

    Article Snippet: Human healthy primary pulmonary arterial endothelial cells (PAECs) were purchased from PromoCell GmbH, Heidelberg, Germany (Cat # C12281).

    Techniques: Expressing